Cell Passaging Cheat Sheet: Just Copy & Paste ✅
Still failing at cell passaging? One diagram to nail it every time ?
Calling all cell culture researchers!!
Over-trypsinization, poor cell adhesion, confusing split ratios, contamination wiping out your entire flask... I've been there ?
This diagram breaks down everything from principles → detailed workflow → pro troubleshooting tips:
⏱️ Digestion time: shorter is safer → Over-trypsinization directly slashes cell viability
? PBS wash must be thorough → Serum residue = trypsin inactivation
? Split ratio depends on cell type → HEK293: 1:3 to 1:6 | Primary cells: 1:2 to 1:3
? Don't max out centrifuge speed → 1000 rpm is enough; higher damages cells
?️ Aseptic technique is non-negotiable → Every second the lid is open = contamination risk
All parameters are labeled. Literally just copy this.
? Save this post, print it for your biosafety hood, and glance at it before every passage. It'll save you years of trial and error ?
Still failing at cell passaging? One diagram to nail it every time ?
Calling all cell culture researchers!!
Over-trypsinization, poor cell adhesion, confusing split ratios, contamination wiping out your entire flask... I've been there ?
This diagram breaks down everything from principles → detailed workflow → pro troubleshooting tips:
⏱️ Digestion time: shorter is safer → Over-trypsinization directly slashes cell viability
? PBS wash must be thorough → Serum residue = trypsin inactivation
? Split ratio depends on cell type → HEK293: 1:3 to 1:6 | Primary cells: 1:2 to 1:3
? Don't max out centrifuge speed → 1000 rpm is enough; higher damages cells
?️ Aseptic technique is non-negotiable → Every second the lid is open = contamination risk
All parameters are labeled. Literally just copy this.
? Save this post, print it for your biosafety hood, and glance at it before every passage. It'll save you years of trial and error ?
